ASSOCIATION OF ANGIOTENSIN CONVERTING ENZYME (ACE) POLYMORPHISM WITH DIABETES IN THE BUEA MUNICIPALITY
|Biochemistry and Molecular Biology|
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|MS word & PDF|
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Diabetes mellitus is a metabolic disorder that is characterized by chromonic hyperglycemia and insulin resistance that arise from the interactions of several genetic and environmental factors. The association of Angiotensin Converting Enzyme (ACE) insertion/deletion (I/D) polymorphism with Type 2 Diabetes Mellitus (T2DM) has been extensively studied throughout various geographic and ethnic populations but with marked inconsistencies.
Thus, this study is aimed at investigating the association of ACE gene polymorphism with diabetes in the Buea municipality.
Identification of specific variations in the Buea municipality will aid in the early diagnosis of pre-diabetics and diabetics, subjects susceptible to diabetes and create awareness in the population about genetic predisposition to T2DM. Blood samples were collected from 11 subjects (5 diabetics and 6 non-diabetics).
DNA was extracted by the non-enzymatic Salting out method and the samples were genotyped for ACE I/D polymorphism by a PCR base method followed with visualization by gel electrophoresis. Upon evaluation of the ACE I/D polymorphism with T2DM, there was a statistically non-significant difference in the frequency of the genotypes (λ²=4.947, p=0.0845). This therefore confirmed that ACE I/D polymorphism may not be associated with T2DM risk in the Buea population.
Diabetes mellitus (DM) is a metabolic disorder characterized by the hyperglycemia with the disturbance of carbohydrate, fats and proteins metabolism that causes defect in insulin secretion, insulin actions or both (Centers for Disease Control and Prevention.,2007).