ASSOCIATION OF ANGIOTENSIN CONVERTING ENZYME (ACE) POLYMORPHISM WITH DIABETES IN THE BUEA MUNICIPALITY

Project Details

Department
Biochemistry and Molecular Biology
Project ID
Bio03
Price
5000XAF
International: $20
No of pages
40
Instruments/method
Empirical 
Reference
YES
Analytical tool
Empirical
Format
 MS word & PDF
Chapters
1-5

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ABSTRACT

Diabetes mellitus is a metabolic disorder that is characterized by chromonic hyperglycemia and insulin resistance that arise from the interactions of several genetic and environmental factors. The association of Angiotensin Converting Enzyme (ACE) insertion/deletion (I/D) polymorphism with Type 2 Diabetes Mellitus (T2DM) has been extensively studied throughout various geographic and ethnic populations but with marked inconsistencies.

Thus, this study is aimed at investigating the association of ACE gene polymorphism with diabetes in the Buea municipality. 

Identification of specific variations in the Buea municipality will aid in the early diagnosis of pre-diabetics and diabetics, subjects susceptible to diabetes and create awareness in the population about genetic predisposition to T2DM. Blood samples were collected from 11 subjects (5 diabetics and 6 non-diabetics).

DNA was extracted by the non-enzymatic Salting out method and the samples were genotyped for ACE I/D polymorphism by a PCR base method followed with visualization by gel electrophoresis. Upon evaluation of the ACE I/D polymorphism with T2DM, there was a statistically non-significant difference in the frequency of the genotypes (λ²=4.947, p=0.0845). This therefore confirmed that ACE I/D polymorphism may not be associated with T2DM risk in the Buea population.

Chapter One

Introduction

Diabetes mellitus (DM) is a metabolic disorder characterized by the hyperglycemia with the disturbance of carbohydrate, fats and proteins metabolism that causes defect in insulin secretion, insulin actions or both (Centers for Disease Control and Prevention.,2007).

1.4 Hypothesis

ACE gene polymorphism is associated with Diabetes Type 2 in the Buea municipality.

1.5 Objectives
1.5.1 Overall Objective:
To investigate the association between ACE gene polymorphism and Diabetes

1.5.2 Specific objectives:

  • To genotype individuals of Buea municipality.
  • To identify genotype responsible for diabetes in Buea.
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